AxL Cleared Tissue LightSheet (AxL CTLS) is a fully automated macro zoom microscope with high NA apochromatic objectives and dual-sided light sheet illumination for ultrafast high-resolution imaging of whole organs to small animals. Custom-designed excitation objectives and patented axially swept light sheet microscopy (ASLM) produce an exceptionally thin, long and uniform lightsheet for large-scale high-resolution imaging. The large field of view of AxL CTLS enables ultrafast imaging of whole organs to small animals. An entire mouse can be prescanned in less than 60 seconds, imaged in 20 minutes and high resolution ASLM scanned in 9 hours revealing neuronal connectivity.