5-Me-dC-CE Phosphoramidite - (10-1060)
5'-Dimethoxytrityl-N-benzoyl-5-methyl-2'-deoxyCytidine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramiditeDescription
C-5 methyl pyrimidine nucleosides are known to stabilize duplexes relative to the non-methylated bases. Therefore, enhanced binding can be achieved using 5-methyl-dC in place of dC, duplex melting temperature being increased by 1.3°. Ac-5-Me-dC-CE Phosphoramidite is fully compatible with AMA deprotection and none of the N4-Me transamination mutant is observed on deprotection. 2,6-Diaminopurine 2’-deoxyriboside (2-amino-dA) forms an additional hydrogen bond with Thymidine, thereby leading to duplex stabilization with a melting temperature increase of 3°. Our 2-amino-dA monomer exhibits fast and effective deprotection in ammonium hydroxide and it is stabilized to depurination during synthesis. Note: we recommend the use of 0.5 M CSO in anhydrous acetonitrile (40-4632-xx) for best results with multiple additions of 2-amino-dA.
Details
Usage
- Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
- Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. Note: Deprotection in AMA (50:50 Ammonium hydroxide/MethylAmine) may result in a 4-5% 5-Methyl-N4-methyl-dC side product at the modification site.
| Specifications | |
|---|---|
| Diluent | Anhydrous Acetonitrile |
| Recommended Storage | Refrigerated storage, maximum of 2-8°C, dry |
| Stability In Solution | Similar to dA,C,G,T-CE Phosphoramidites |
Dilution/Coupling Data
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.
ABI 392/394
| Catalog # | Pack Size | Grams/Pack | 0.1M Dil. (mL) | Approximate Number of Additions | |||||
|---|---|---|---|---|---|---|---|---|---|
| LV40 | LV200 | 40nm | 0.2μm | 1μm | 10μm | ||||
| 10-1060-02 | 0.25 g | 0.25grams | 2.95 | 85 | 51 | 31.88 | 23.18 | 17 | 4.25 |
| 10-1060-05 | 0.5 g | 0.5grams | 5.9 | 183.3 | 110 | 68.7 | 50 | 36.6 | 9.1 |
| 10-1060-10 | 1.0 g | 1.0grams | 11.79 | 379.6 | 227.8 | 142.3 | 103.5 | 75.9 | 18.9 |
Expedite
| Catalog # | Pack Size | Grams/Pack | Dilution (mL) | Approximate Number of Additions | ||||
|---|---|---|---|---|---|---|---|---|
| Molarity | 50nm | 0.2μm | 1μm | 15μm | ||||
| 10-1060-02 | 0.25 g | 0.25grams | 4.4 | 0.07 | 81.6 | 51 | 37.09 | 5.1 |
| 10-1060-05 | 0.5 g | 0.5grams | 8.8 | 0.07 | 169.6 | 106 | 77 | 10.6 |
| 10-1060-10 | 1.0 g | 1.0grams | 17.6 | 0.07 | 345.6 | 216 | 157 | 21.6 |
Technical documents
Certificate of analysis
CoA search tool
References