Abstract
Glutathione (GSH; L- -glutamyl-L-cysteinl-glycine) plays an important role in the prevention of radical mediated injury to the body. It does so as a radical scavenger and by supplying GSH to the antioxidant enzymes described in Chapter 29. In conjunction with superoxide dismutase (SOD), which converts superoxide anions into hydrogen peroxide (H202), glutathione peroxidase (GSHPx) converts H202, into water (1). As a result of the second conversion, GSH is oxidized to glutathione disulfide (GSSG). In this way, GSH acts as a cofactor in the removal of toxic radicals from the body. During oxidative stress, GSH levels decline and GSSG increases, which can influence signal transduction by stimulating NF-kB activation (2). GSH is also thought to be a donor of glutamyl groups in amino-acid transport (3).
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References
Southorn, P A and Powis, G (1988) Mayo Clin. Proc 63, 381–389
Droge, W, Schulze-Orthoff, K., Mihm, S., Gaiter, D., Schenk, H, Eck, H P., Roth, S., and Gmunder, H (1994) Function of glutathione and glutathione disulfide in immunology and immunopathology. FASEB-J 8, 1131–1138
Orlowski, M. and Meister, A (1970) Proc Nat. Acad. Sci. USA, 67, 1248–1255.
Bhattacharya, S K, Robson, J. S, and Steward, C. P (1955) The determination of glutathione in blood and tissue. Biochem J. 60, 696–702.
Boyd, S C., Sasame, H. A., and Boyd, M. R. (1979) High concentrations of glutathione in glandular stomach: possible implications for carcinogenesis Science 205, 1010–1012
Hissin, P. J. and Hilf, R. (1976) Anal Biochem. 74, 214–226.
Coutelle, C., Iron, A, Higueret, D, and Cassaigne, A (1992) Optimization of a spectrophotometry assay of total and oxidized blood glutathione comparison with a fluorometric method Ann Biol. Clin. Paris. 50, 71–76.
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© 1998 Humana Press Inc., Totowa, NJ
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Browne, R.W., Armstrong, D. (1998). Reduced Glutathione and Glutathione Disulfide. In: Armstrong, D. (eds) Free Radical and Antioxidant Protocols. Methods in Molecular Biology™, vol 108. Humana Press. https://doi.org/10.1385/0-89603-472-0:347
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DOI: https://doi.org/10.1385/0-89603-472-0:347
Publisher Name: Humana Press
Print ISBN: 978-0-89603-472-3
Online ISBN: 978-1-59259-254-8
eBook Packages: Springer Protocols
Keywords
- Volumetric Flask
- Nicotinamide Adenine Dinucleotide Phosphate
- Glass Test Tube
- Glutathione Disulfide
- Disodium Ethylenediamine Tetraacetic Acid
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