| O/S | Outside | |
| O/S | Outstanding (unpaid insurance claim) | |
| O/S | Offset | |
|
| O/S | Overseas | |
| O/S | on sale | |
| O/S | Out of State | |
| OS | Operating System | |
| OS | Open Source | |
| OS | Open Systems | |
| O/S | One Size | |
| OS | Overseas | |
| OS | Oversize | |
| OS | Old School | |
|
| OS | Online Services | |
| OS | Over Stock | |
| OS | One Side | |
| OS | Overshoot | |
| OS | Overall Survival | |
| O/S | On Scene | |
| OS | On-Site | |
| OS | Off Screen (cinematography) | |
| OS | Outer Space | |
| OS | Out of Stock | |
| O/S | Operating System | |
| OS | Office of the Secretary | |
| OS | Operational Security | |
| OS | Office Support | |
| O/S | Operational/Support | |
| OS | Oxidative Stress | |
| OS | Old Style | |
| OS | Old Style (or Old System; referring to Julian Calendars) | |
| OS | Over the Shoulder (cinematography) | |
| OS | Oxygen Sensor | |
| OS | Ordnance Survey | |
| OS | On Scene | |
| OS | Old Stuff :-) | |
| OS | Occupational Safety (varios organizations) | |
| OS | Oregon State (also seen as OSU) | |
| OS | Over Speed | |
| Os | Orioles (Baltimore baseball team) | |
| OS | Omaha Steaks (meat company) | |
| OS | Orlando Sentinel (Orlando, FL newspaper) | |
| OS | On Schedule | |
| OS | Ordinary Seaman | |
| OS | Ordinary Seaman (Canadian naval rank) | |
| OS | Outlaw Star (action-anime) | |
| OS | Office of Systems | |
| OS | Old Saxon | |
| OS | Os Giken (automotive racing parts manufacturer) | |
| OS | Obiettivi Specifici | |
| OS | Outsourcing Services | |
| OS | Output Status | |
| O/S | Over Stock | |
| OS | Official Statement (government finances) | |
| OS | Our Savior | |
| OS | Over Shield (Halo video game) | |
| OS | Austrian Airlines (IATA airline code) | |
| OS | Ocean Springs (Mississippi) | |
| OS | Operations Support (various organizations) | |
| OS | Obsidian Sanctum (World of Warcraft) | |
| OS | Oliver Stone (director) | |
| OS | Organized Stalking | |
| OS | Oberseminar | |
| OS | Osmium | |
| OS | Operator Station | |
| OS | Old Spice (after shave brand) | |
| O/S | Oversize (Canada Post) | |
| OS | Oblates of Wisdom | |
| OS | Ocean Shores (Washington) | |
| OS | Out-of-Service | |
| OS | Overall Satisfaction | |
| OS | Operationalizing the Strategy | |
| OS | Owen Sound (City, Canada) | |
| OS | Operator Services (various agencies) | |
| OS | Off Stage | |
| OS | Operserv (IRC Operator Server) | |
| os | Ossetian (language identifier; RFC 3066) | |
| OS | Oculus Sinister (Latin: Left Eye) | |
| OS | Oversold (stock market) | |
| OS | Only Son | |
| OS | Observational Study | |
| OS | Orthopedic Surgeon | |
| OS | Operations Specialist (USN Rating) | |
| OS | Ouabain-Sensitive | |
| OS | Open Sockets (Diablo II gaming) | |
| OS | Official Solicitor (UK) | |
| O/S | Ornstein & Smough (gaming; Dark Souls) | |
| OS | Optical Scanning | |
| OS | Ouvrier Spécialisé (French: semi-skilled worker) | |
| OS | Olympiska Spelen (Swedish: Olympic Games) | |
| OS | Ohr Somayach (school) | |
| O/S | Outstanding Shares (stock market) | |
| OS | Oh Shoot (polite form) | |
| OS | Own Ship | |
| OS | Outdoor Skills | |
| OS | Output Spy | |
| OS | Osijek, Croatia (license plate) | |
| OS | Outstation | |
| OS | Orthopterists' Society | |
| OS | Ogawa Shigeo (Engines) | |
| OS | Organizational Support | |
| OS | Onboard Software | |
| OS | Observation Squadron | |
| OS | Ocean Surveillance | |
| OS | Off Scene | |
| OS | Office of Security | |
| OS | Office Superintendent | |
| O/S | Outstation | |
| OS | Operations Squadron | |
| OS | Order Statistic | |
| OS | Orbital Station | |
| OS | Oil Separator | |
| OS | Office of Sustainability | |
| O/S | Outlaw Shark | |
| OS | Orbiting Sample (space flight) | |
| OS | Operating Standard | |
| OS | Operating Statement | |
| O/S | Organisation/Schmelt (Germany/Poland) | |
| O/S | Ocean Surveillance | |
| OS | Oughtred Society (est. 1991; slide rule collectors) | |
| OS | Opening Snap (mitral valve) | |
| OS | Offensive Support | |
| OS | Optical Sight | |
| OS | Otm Shank Jazz Quintet (band) | |
| OS | Open Skies treaty | |
| OS | Orienting Station (surveyed reference location for artillery position) | |
| OS | Ordnance Specification | |
| OS | Officer's Statement (performance evaluation) | |
| OS | Operational Suitability | |
| OS | Object Slice | |
| OS | Operating Strength | |
| OS | Operational Superintendence (on overtime forms) | |
| OS | Optical Silicon (semiconductor chip components) | |
| OS | Overtime Scheduled | |
| OS | Old Silcoatian (Silcoates School, Wakefield, UK) | |
| OS | Organic Signal | |